Features
The Arolab laboratory (90 m2 approx. ) at IB-UMH includes seven workstations on a high floor, and is equipped with essential equipment for: (i) the preparation of plant media (two precision balances, four refrigerators, a thermostatic bath, a pH meter, two magnetic stirrers and a microwave); (ii) the use of molecular biology techniques (one -80°C upright freezer, one -20°C chest freezer, five refrigerators, seven sets of micropipettes, several single-channel electronic pipettes, two thermoblocks, three benchtop microcentrifuges, one thermal cycler, one gel documentation system, several electrophoresis tanks, two electrophoresis power supplies, one UV/Vis spectrophotometer and one electroporation system); and (iii) phenotypic analysis (three stereomicroscopes, a trinocular microscope with digital image capture system and a fluorescence binocular microscope). We also have office space of about 15 m2 equipped with five computers, three laptops, a flatbed scanner, two laser printers and a web server for data storage.
We have a Plant Tissue Culture Room (23 m2) with two horizontal laminar flow cabinets, an autoclave, an incubator with orbital shaking and heating, two culture ovens, a high capacity refrigerated centrifuge (up to 2.6 L volume) and three culture cabinets for germination and plant tissue culture (one Panasonic MLR 352 PE and two adapted food cabinets). Two visitable phytotrons (16 m3 approx. each) are available on the second floor of the building for growing tomato plants. In 2019 we obtained funding for the purchase of a ZEISS Lightsheet Z.1 LSFM microscope equipped with three laser diodes (405, 488, 561 nm) (IDIFEDER/2018/016), which allows us to acquire time-resolved images of plant tissues over extended periods of time. We have recently joined two infrastructure projects led by Prof. J.L. Micol, EQC2018-005181-P and IDIFEDER/2021/033, which makes available to our research group a NextSeq 550 System (Illumina), and a super-resolution confocal microscope.
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